Genetic alterations of <i>KRAS</i> and <i>TP53</i> in intrahepatic cholangiocarcinoma associated with poor prognosis

Abstract The aim of this study is to investigate certain genetic features intrahepatic cholangiocarcinoma (ICCA). A total 12 eligible ICCA patients were enrolled, and tumor tissues from the subjected next-generation sequencing a multi-genes panel. Tumor mutation burden (TMB), mutated genes, copy number variants (CNVs), pathway enrichment analysis performed. median TMB was 2.76 Mutation/Mb (range, 0–36.62 Mutation/Mb) in patients. top two most commonly genes KRAS (33%) TP53 (25%). co-mutations 16.7% (2/12) Notably, patient P6 with highest did not have mutations. Additionally, and/or alterations significantly associated poor progression-free survival than those wild type (1.4 months vs 18 months). DNA damage repair homologs recombinant deficiencies high cases. In conclusion, we found that mutations could predict prognosis